1DB1

CRYSTAL STRUCTURE OF THE NUCLEAR RECEPTOR FOR VITAMIN D COMPLEXED TO VITAMIN D

1DB1 の概要

• エントリーDOI: 10.2210/pdb1db1/pdb
• 分子名称: VITAMIN D NUCLEAR RECEPTOR, 5-{2-[1-(5-HYDROXY-1,5-DIMETHYL-HEXYL)-7A-METHYL-OCTAHYDRO-INDEN-4-YLIDENE]-ETHYLIDENE}-4-METHYLENE-CYCLOHEXANE-1,3-DIOL (3 entities in total)
• 機能のキーワード: complex, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P11473
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29808.51

構造登録者

Rochel, N.,Wurtz, J.M.,Mitschler, A.,Klaholz, B.,Moras, D. (登録日: 1999-11-02, 公開日: 2000-01-31, 最終更新日: 2024-02-07)

主引用文献

Rochel, N.,Wurtz, J.M.,Mitschler, A.,Klaholz, B.,Moras, D.
The crystal structure of the nuclear receptor for vitamin D bound to its natural ligand.
Mol.Cell, 5:173-179, 2000

PubMed Abstract: The action of 1 alpha, 25-dihydroxyvitamin D3 is mediated by its nuclear receptor (VDR), a ligand-dependent transcription regulator. We report the 1.8 A resolution crystal structure of the complex between a VDR ligand-binding domain (LBD) construct lacking the highly variable VDR-specific insertion domain and vitamin D. The construct exhibits the same binding affinity for vitamin D and transactivation ability as the wild-type protein, showing that the N-terminal part of the LBD is essential for its structural and functional integrity while the large insertion peptide is dispensable. The structure reveals the active conformation of the bound ligand and allows understanding of the different binding properties of some synthetic analogs.

PubMed: 10678179
DOI: 10.1016/S1097-2765(00)80413-X

実験手法

X-RAY DIFFRACTION (1.8 Å)