1D66

DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX

Summary for 1D66

• Entry DOI: 10.2210/pdb1d66/pdb
• Descriptor: DNA (5'-D(*CP*CP*GP*GP*AP*GP*GP*AP*CP*AP*GP*TP*CP*CP*TP*CP*C P*GP*G)-3'), DNA (5'-D(*CP*CP*GP*GP*AP*GP*GP*AP*CP*TP*GP*TP*CP*CP*TP*CP*C P*GP*G)-3'), PROTEIN (GAL4), ... (5 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Nucleus: P04386
• Total number of polymer chains: 4
• Total formula weight: 27736.86

Authors

Marmorstein, R.,Carey, M.,Ptashne, M.,Harrison, S.C. (deposition date: 1992-03-06, release date: 1992-03-06, Last modification date: 2024-02-07)

Primary citation

Marmorstein, R.,Carey, M.,Ptashne, M.,Harrison, S.C.
DNA recognition by GAL4: structure of a protein-DNA complex.
Nature, 356:408-414, 1992

PubMed Abstract: A specific DNA complex of the 65-residue, N-terminal fragment of the yeast transcriptional activator, GAL4, has been analysed at 2.7 A resolution by X-ray crystallography. The protein binds as a dimer to a symmetrical 17-base-pair sequence. A small, Zn(2+)-containing domain recognizes a conserved CCG triplet at each end of the site through direct contacts with the major groove. A short coiled-coil dimerization element imposes 2-fold symmetry. A segment of extended polypeptide chain links the metal-binding module to the dimerization element and specifies the length of the site. The relatively open structure of the complex would allow another protein to bind coordinately with GAL4.

PubMed: 1557122
DOI: 10.1038/356408a0

Experimental method

X-RAY DIFFRACTION (2.7 Å)