1D66

DNA RECOGNITION BY GAL4: STRUCTURE OF A PROTEIN/DNA COMPLEX

1D66 の概要

• エントリーDOI: 10.2210/pdb1d66/pdb
• 分子名称: DNA (5'-D(*CP*CP*GP*GP*AP*GP*GP*AP*CP*AP*GP*TP*CP*CP*TP*CP*C P*GP*G)-3'), DNA (5'-D(*CP*CP*GP*GP*AP*GP*GP*AP*CP*TP*GP*TP*CP*CP*TP*CP*C P*GP*G)-3'), PROTEIN (GAL4), ... (5 entities in total)
• 機能のキーワード: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P04386
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27736.86

構造登録者

Marmorstein, R.,Carey, M.,Ptashne, M.,Harrison, S.C. (登録日: 1992-03-06, 公開日: 1992-03-06, 最終更新日: 2024-02-07)

主引用文献

Marmorstein, R.,Carey, M.,Ptashne, M.,Harrison, S.C.
DNA recognition by GAL4: structure of a protein-DNA complex.
Nature, 356:408-414, 1992

PubMed Abstract: A specific DNA complex of the 65-residue, N-terminal fragment of the yeast transcriptional activator, GAL4, has been analysed at 2.7 A resolution by X-ray crystallography. The protein binds as a dimer to a symmetrical 17-base-pair sequence. A small, Zn(2+)-containing domain recognizes a conserved CCG triplet at each end of the site through direct contacts with the major groove. A short coiled-coil dimerization element imposes 2-fold symmetry. A segment of extended polypeptide chain links the metal-binding module to the dimerization element and specifies the length of the site. The relatively open structure of the complex would allow another protein to bind coordinately with GAL4.

PubMed: 1557122
DOI: 10.1038/356408a0

実験手法

X-RAY DIFFRACTION (2.7 Å)