1D3U

TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/BRE+TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI

Summary for 1D3U

• Entry DOI: 10.2210/pdb1d3u/pdb
• Descriptor: DNA 24-MER: BRE+TATA-BOX, DNA 23-MER: BRE+TATA-BOX, TATA-BINDING PROTEIN, ... (5 entities in total)
• Functional Keywords: protein-dna complex, transcription, hyperthermophile, gene regulation-dna complex, gene regulation/dna
• Biological source: Pyrococcus woesei; More
• Total number of polymer chains: 4
• Total formula weight: 57306.55

Authors

Littlefield, O.,Korkhin, Y.,Sigler, P.B. (deposition date: 1999-10-01, release date: 1999-11-15, Last modification date: 2024-10-30)

Primary citation

Littlefield, O.,Korkhin, Y.,Sigler, P.B.
The structural basis for the oriented assembly of a TBP/TFB/promoter complex.
Proc.Natl.Acad.Sci.USA, 96:13668-13673, 1999

PubMed Abstract: Recently the definition of the metazoan RNA polymerase II and archaeal core promoters has been expanded to include a region immediately upstream of the TATA box called the B recognition element (BRE), so named because eukaryal transcription factor TFIIB and its archaeal orthologue TFB interact with the element in a sequence-specific manner. Here we present the 2.4-A crystal structure of archaeal TBP and the C-terminal core of TFB (TFB(c)) in a complex with an extended TATA-box-containing promoter that provides a detailed picture of the stereospecific interactions between the BRE and a helix-turn-helix motif in the C-terminal cyclin repeat of TFB(c). This interaction is important in determining the level of basal transcription and explicitly defines the direction of transcription.

PubMed: 10570130
DOI: 10.1073/pnas.96.24.13668

Experimental method

X-RAY DIFFRACTION (2.4 Å)