1D29

CRYSTAL STRUCTURE ANALYSIS OF THE B-DNA DODECAMER CGTGAATTCACG

Summary for 1D29

• Entry DOI: 10.2210/pdb1d29/pdb
• Descriptor: DNA (5'-D(*CP*GP*TP*GP*AP*AP*TP*TP*CP*AP*CP*G)-3') (2 entities in total)
• Functional Keywords: b-dna, double helix, dna
• Total number of polymer chains: 2
• Total formula weight: 7324.81

Authors

Larsen, T.A.,Kopka, M.L.,Dickerson, R.E. (deposition date: 1991-05-23, release date: 1992-04-15, Last modification date: 2024-02-07)

Primary citation

Larsen, T.A.,Kopka, M.L.,Dickerson, R.E.
Crystal structure analysis of the B-DNA dodecamer CGTGAATTCACG.
Biochemistry, 30:4443-4449, 1991

PubMed Abstract: The crystal structure of the DNA dodecamer C-G-T-G-A-A-T-T-C-A-C-G has been determined at a resolution of 2.5 A, with a final R factor of 15.8% for 1475 nonzero reflections measured at 0 degrees C. The structure is isomorphous with that of the Drew dodecamer, with the space group P2(1)2(1)2(1) and cell dimensions of a = 24.94 A, b = 40.78 A, and c = 66.13 A. The asymmetric unit contains all 12 base pairs of the B-DNA double helix and 36 water molecules. The structure of C-G-T-G-A-A-T-T-C-A-C-G is very similar to that of C-G-C-G-A-A-T-T-C-G-C-G, with no major alterations in helix parameters. Water peaks in the refined structure appear to represent a selection of peaks that were observed in the Drew dodecamer. The minor-groove spine of hydration at 2.5 A is fragmentary, but as Narendra et al. (1991) [Biochemistry (following paper in this issue)] have observed, lowering the temperature leads to a more complete representation of the spine.

PubMed: 1850624
DOI: 10.1021/bi00232a010

Experimental method

X-RAY DIFFRACTION (2.5 Å)