1D0B

INTERNALIN B LEUCINE RICH REPEAT DOMAIN

Summary for 1D0B

• Entry DOI: 10.2210/pdb1d0b/pdb
• Descriptor: INTERNALIN B, CALCIUM ION (3 entities in total)
• Functional Keywords: leucine rich repeat, calcium binding, cell adhesion
• Biological source: Listeria monocytogenes
• Total number of polymer chains: 1
• Total formula weight: 23836.43

Authors

Marino, M.,Braun, L.,Cossart, P.,Ghosh, P. (deposition date: 1999-09-09, release date: 2000-01-07, Last modification date: 2024-02-07)

Primary citation

Marino, M.,Braun, L.,Cossart, P.,Ghosh, P.
Structure of the lnlB leucine-rich repeats, a domain that triggers host cell invasion by the bacterial pathogen L. monocytogenes.
Mol.Cell, 4:1063-1072, 1999

PubMed Abstract: The L. monocytogenes protein lnlB activates phosphoinositide 3-kinase and induces phagocytosis in several mammalian cell types. The 1.86 A resolution X-ray crystal structure of the leucine-rich repeat domain of lnlB that is both necessary and sufficient to induce phagocytosis is presented here. The structure supports a crucial role for calcium in host cell invasion by L. monocytogenes and supplies a rationale for its function. Calciums are bound to the protein in an unusually exposed manner that suggests that the metals may act as a bridge between lnlB and mammalian cell surface receptors. The structure also identifies surfaces on the curved and elongated molecule that may constitute additional interaction sites in forming a bacterial-mammalian signaling complex.

PubMed: 10635330
DOI: 10.1016/S1097-2765(00)80234-8

Experimental method

X-RAY DIFFRACTION (1.86 Å)