1CYX

QUINOL OXIDASE (PERIPLASMIC FRAGMENT OF SUBUNIT II WITH ENGINEERED CU-A BINDING SITE)(CYOA)

1CYX の概要

• エントリーDOI: 10.2210/pdb1cyx/pdb
• 分子名称: CYOA, DINUCLEAR COPPER ION (3 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cell inner membrane; Multi-pass membrane protein: P0ABJ1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22869.84

構造登録者

Wilmanns, M.,Lappalainen, P.,Kelly, M.,Sauer-Eriksson, E.,Saraste, M. (登録日: 1995-08-22, 公開日: 1996-03-08, 最終更新日: 2024-02-07)

主引用文献

Wilmanns, M.,Lappalainen, P.,Kelly, M.,Sauer-Eriksson, E.,Saraste, M.
Crystal structure of the membrane-exposed domain from a respiratory quinol oxidase complex with an engineered dinuclear copper center.
Proc.Natl.Acad.Sci.USA, 92:11955-11959, 1995

PubMed Abstract: Cytochrome oxidase is a membrane protein complex that catalyzes reduction of molecular oxygen to water and utilizes the free energy of this reaction to generate a transmembrane proton gradient during respiration. The electron entry site in subunit II is a mixed-valence dinuclear copper center in enzymes that oxidize cytochrome c. This center has been lost during the evolution of the quinoloxidizing branch of cytochrome oxidases but can be restored by engineering. Herein we describe the crystal structures of the periplasmic fragment from the wild-type subunit II (CyoA) of Escherichia coli quinol oxidase at 2.5-A resolution and of the mutant with the engineered dinuclear copper center (purple CyoA) at 2.3-A resolution. CyoA is folded as an 11-stranded mostly antiparallel beta-sandwich followed by three alpha-helices. The dinuclear copper center is located at the loops between strands beta 5-beta 6 and beta 9-beta 10. The two coppers are at a 2.5-A distance and symmetrically coordinated to the main ligands that are two bridging cysteines and two terminal histidines. The residues that are distinct in cytochrome c and quinol oxidases are around the dinuclear copper center. Structural comparison suggests a common ancestry for subunit II of cytochrome oxidase and blue copper-binding proteins.

PubMed: 8618822
DOI: 10.1073/pnas.92.26.11955

実験手法

X-RAY DIFFRACTION (2.3 Å)