1CX7

T4 LYSOZYME METHIONINE CORE MUTANT

1CX7 の概要

• エントリーDOI: 10.2210/pdb1cx7/pdb
• 分子名称: LYSOZYME, CHLORIDE ION, 2-HYDROXYETHYL DISULFIDE, ... (4 entities in total)
• 機能のキーワード: hydrolase (o-glycosyl), t4 lysozyme, methionine core mutant, protein engineering, protein folding, hydrolase
• 由来する生物種: Enterobacteria phage T4
• 細胞内の位置: Host cytoplasm : P00720
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18945.77

構造登録者

Gassner, N.C.,Baase, W.A.,Lindstrom, J.,Lu, J.,Matthews, B.W. (登録日: 1999-08-28, 公開日: 1999-11-10, 最終更新日: 2024-02-07)

主引用文献

Gassner, N.C.,Matthews, B.W.
Use of differentially substituted selenomethionine proteins in X-ray structure determination.
Acta Crystallogr.,Sect.D, 55:1967-1970, 1999

PubMed Abstract: Using heavily methionine-substituted T4 lysozyme as an example, it is shown how the addition or deletion of a small number of methionines can simplify the location of selenium sites for use in MAD phasing. By comparing the X-ray data for a large number of singly substituted lysozymes, it is shown that the optimal amino acid to be substituted by methionine is leucine, followed, in order of preference, by phenylalanine, isoleucine and valine. The identification of leucine as the first choice agrees with the ranking suggested by the Dayhoff mutation probability, i.e. by the frequency of amino-acid substitutions in the sequences of related proteins. The ranking of the second and subsequent choices, however, differ significantly.

PubMed: 10666571
DOI: 10.1021/bi9915519

実験手法

X-RAY DIFFRACTION (1.94 Å)