1CW0

CRYSTAL STRUCTURE ANALYSIS OF VERY SHORT PATCH REPAIR (VSR) ENDONUCLEASE IN COMPLEX WITH A DUPLEX DNA

1CW0 の概要

• エントリーDOI: 10.2210/pdb1cw0/pdb
• 分子名称: DNA (5'-D(*AP*CP*GP*TP*AP*CP*CP*TP*GP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*C)-3'), DNA (5'-D(P*TP*AP*GP*GP*TP*AP*CP*GP*T)-3'), ... (7 entities in total)
• 機能のキーワード: protein-dna complex, mismatch, intercalation, zinc, hydrolase/dna, hydrolase-dna complex
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 25324.34

構造登録者

Tsutakawa, S.E.,Jingami, H.,Morikawa, K. (登録日: 1999-08-25, 公開日: 1999-12-12, 最終更新日: 2023-08-09)

主引用文献

Tsutakawa, S.E.,Jingami, H.,Morikawa, K.
Recognition of a TG mismatch: the crystal structure of very short patch repair endonuclease in complex with a DNA duplex.
Cell(Cambridge,Mass.), 99:615-623, 1999

PubMed Abstract: The crystal structure of very short patch repair (Vsr) endonuclease, in complex with Mg2+ and with duplex DNA containing a TG mismatch, has been determined at 2.3 A resolution. In E. coli, the enzyme recognizes a TG mismatched base pair, generated after spontaneous deamination of methylated cytosines, and cleaves the phosphate backbone on the 5' side of the thymine. Extensive interactions between the DNA and the protein characterize a novel recognition mechanism, where three aromatic residues intercalate from the major groove into the DNA to strikingly deform the base pair stacking. With the presence of a cleaved DNA intermediate in the active center, the structure of the Vsr/DNA complex provides detailed insights into the catalytic mechanism for endonuclease activity.

PubMed: 10612397
DOI: 10.1016/S0092-8674(00)81550-0

実験手法

X-RAY DIFFRACTION (2.3 Å)