1CVL

CRYSTAL STRUCTURE OF BACTERIAL LIPASE FROM CHROMOBACTERIUM VISCOSUM ATCC 6918

1CVL の概要

• エントリーDOI: 10.2210/pdb1cvl/pdb
• 分子名称: TRIACYLGLYCEROL HYDROLASE, CALCIUM ION (3 entities in total)
• 機能のキーワード: triacylglycerol-hydrolase, pseudomonadaceae, oxyanion, cis-peptide, hydrolase
• 由来する生物種: Chromobacterium viscosum
• 細胞内の位置: Secreted: Q05489
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33157.78

構造登録者

Lang, D.A.,Hofmann, B.,Haalck, L.,Hecht, H.-J.,Spener, F.,Schmid, R.D.,Schomburg, D. (登録日: 1997-01-09, 公開日: 1997-04-01, 最終更新日: 2024-10-30)

主引用文献

Lang, D.,Hofmann, B.,Haalck, L.,Hecht, H.J.,Spener, F.,Schmid, R.D.,Schomburg, D.
Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution.
J.Mol.Biol., 259:704-717, 1996

PubMed Abstract: The crystal structure of a lipase from the bacterium Chromobacterium viscosum ATCC 6918 (CVL) has been determined by isomorphous replacement and refined at 1.6 angstroms resolution to an R-factor of 17.8%. The lipase has the overall topology of an alpha/beta type protein, which was also found for previously determined lipase structures. The catalytic triad of the active center consists of the residues Ser87, Asp263 and His285. These residues are not exposed to the solvent, but a narrow channel connects them with the molecular surface. This conformation is very similar to the previously reported closed conformation of Pseudomonas glumae lipase (PGL), but superposition of the two lipase structures reveals several conformational differences. r.m.s. deviations greater than 2 angstroms are found for the C alpha-atoms of the polypeptide chains from His15 to Asp28, from Leu49 to Ser54 and from Lys128 to Gln158. Compared to the PGL structure in the CVL structure, three alpha-helical fragments are shorter, one beta-strand is longer and an additional antiparallel beta-sheet is found. In contrast to PGL, CVL displays an oxyanion hole, which is stabilized by the amide nitrogen atoms of Leu17 and Gln88, and a cis-peptide bond between Gln291 and Leu292. CVL contains a Ca2+, like the PGL, which is coordinated by four oxygen atoms from the protein and two water molecules.

PubMed: 8683577
DOI: 10.1006/jmbi.1996.0352

実験手法

X-RAY DIFFRACTION (1.6 Å)