1CUO

CRYSTAL STRUCTURE ANALYSIS OF ISOMER-2 AZURIN FROM METHYLOMONAS J

1CUO の概要

• エントリーDOI: 10.2210/pdb1cuo/pdb
• 分子名称: PROTEIN (AZURIN ISO-2), COPPER (II) ION (3 entities in total)
• 機能のキーワード: beta barrel, periplasmic, electron transport
• 由来する生物種: Methylomonas sp. J
• 細胞内の位置: Periplasm: P12335
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13886.09

構造登録者

Inoue, T.,Nishio, N.,Kai, Y.,Suzuki, S.,Kataoka, K. (登録日: 1999-08-21, 公開日: 2000-08-23, 最終更新日: 2024-10-16)

主引用文献

Inoue, T.,Suzuki, S.,Nishio, N.,Yamaguchi, K.,Kataoka, K.,Tobari, J.,Yong, X.,Hamanaka, S.,Matsumura, H.,Kai, Y.
The significance of the flexible loop in the azurin (Az-iso2) from the obligate methylotroph Methylomonas sp. strain J.
J.Mol.Biol., 333:117-124, 2003

PubMed Abstract: The obligate methylotroph Methylomonas sp. strain J produces two azurins (Az-iso1 and Az-iso2) as candidates for electron acceptor from methylamine dehydrogenase (MADH) in the electron-transfer process involving the oxidation of methylamine to formaldehyde and ammonia. The X-ray crystallographic study indicated that Az-iso2 gives two types of crystals (form I and form II) with polyethylene glycol (PEG4000) and ammonium sulfate as the precipitants, respectively. Comparison between the two Az-iso2 structures in forms I and II reveals the remarkable structural changes at the top surface of the molecule around the copper atom. Az-iso2 possesses Gly43 instead of Val43 or Ala43, which is unique among all other azurins around the copper ligand His46, inducing the remarkable structural change in the loop region from Gly37 to Gly43. When the structure of Az-iso2 is superimposed on that of amicyanin in the ternary complex composed of MADH, amicyanin, and cytochrome c(551), the loop of Az-iso2 deeply overlaps with the light subunit of MADH. However, the Az-iso2 molecule is probably able to avoid any steric hindrance with the cognate MADH to form the complex for intermolecular electron-transfer reaction, since the loop containing Gly43 is flexible. We discuss why the electron-transfer activity of Az-iso2 is fivefold higher than that of Az-iso1.

PubMed: 14516747
DOI: 10.1016/j.jmb.2003.08.002

実験手法

X-RAY DIFFRACTION (1.6 Å)