1CUK

ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE

1CUK の概要

• エントリーDOI: 10.2210/pdb1cuk/pdb
• 分子名称: RUVA PROTEIN (2 entities in total)
• 機能のキーワード: dna repair, sos response, dna-binding, dna recombination, helicase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22197.78

構造登録者

Rafferty, J.B.,Rice, D.W. (登録日: 1996-08-28, 公開日: 1997-10-22, 最終更新日: 2024-02-07)

主引用文献

Rafferty, J.B.,Sedelnikova, S.E.,Hargreaves, D.,Artymiuk, P.J.,Baker, P.J.,Sharples, G.J.,Mahdi, A.A.,Lloyd, R.G.,Rice, D.W.
Crystal structure of DNA recombination protein RuvA and a model for its binding to the Holliday junction.
Science, 274:415-421, 1996

PubMed Abstract: The Escherichia coli DNA binding protein RuvA acts in concert with the helicase RuvB to drive branch migration of Holliday intermediates during recombination and DNA repair. The atomic structure of RuvA was determined at a resolution of 1.9 angstroms. Four monomers of RuvA are related by fourfold symmetry in a manner reminiscent of a four-petaled flower. The four DNA duplex arms of a Holliday junction can be modeled in a square planar configuration and docked into grooves on the concave surface of the protein around a central pin that may facilitate strand separation during the migration reaction. The model presented reveals how a RuvAB-junction complex may also accommodate the resolvase RuvC.

PubMed: 8832889
DOI: 10.1126/science.274.5286.415

実験手法

X-RAY DIFFRACTION (1.9 Å)