1CTO

NMR STRUCTURE OF THE C-TERMINAL DOMAIN OF THE LIGAND-BINDING REGION OF MURINE GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR, MINIMIZED AVERAGE STRUCTURE

1CTO の概要

• エントリーDOI: 10.2210/pdb1cto/pdb
• 分子名称: GRANULOCYTE COLONY-STIMULATING FACTOR RECEPTOR (1 entity in total)
• 機能のキーワード: binding protein, cytokine receptor
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P40223
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12536.45

構造登録者

Yamasaki, K.,Naito, S.,Anaguchi, H.,Ohkubo, T.,Ota, Y. (登録日: 1996-09-25, 公開日: 1997-10-22, 最終更新日: 2024-10-23)

主引用文献

Yamasaki, K.,Naito, S.,Anaguchi, H.,Ohkubo, T.,Ota, Y.
Solution structure of an extracellular domain containing the WSxWS motif of the granulocyte colony-stimulating factor receptor and its interaction with ligand.
Nat.Struct.Biol., 4:498-504, 1997

PubMed Abstract: We have determined the NMR structure of a ligand-binding domain of the granulocyte colony-stimulating factor (G-CSF) receptor, containing the highly conserved WSxWS motif. The domain consists of seven beta-strands with the fibronectin type III-like topology seen in several cytokine receptors. Comparisons between the spectra of the 15N-labelled domain with and without G-CSF indicate that the major ligand-recognition site is on the FG loop just upstream of the WSxWS sequence, and not on the BC loop which is mainly used in the growth hormone system. The WSxWS residues are suggested to contribute to ligand-recognition and to the protein architecture of the G-CSF receptor.

PubMed: 9187659
DOI: 10.1038/nsb0697-498

実験手法

SOLUTION NMR