1CSG

Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor

Summary for 1CSG

• Entry DOI: 10.2210/pdb1csg/pdb
• Descriptor: Granulocyte-macrophage colony-stimulating factor (1 entity in total)
• Functional Keywords: cytokine
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P04141
• Total number of polymer chains: 2
• Total formula weight: 28984.99

Authors

Walter, M.R.,Cook, W.J.,Ealick, S.E. (deposition date: 1992-11-23, release date: 1993-10-31, Last modification date: 2024-11-20)

Primary citation

Walter, M.R.,Cook, W.J.,Ealick, S.E.,Nagabhushan, T.L.,Trotta, P.P.,Bugg, C.E.
Three-dimensional structure of recombinant human granulocyte-macrophage colony-stimulating factor.
J.Mol.Biol., 224:1075-1085, 1992

PubMed Abstract: The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.

PubMed: 1569568
DOI: 10.1016/0022-2836(92)90470-5

Experimental method

X-RAY DIFFRACTION (2.7 Å)