1COO

THE COOH-TERMINAL DOMAIN OF RNA POLYMERASE ALPHA SUBUNIT

1COO の概要

• エントリーDOI: 10.2210/pdb1coo/pdb
• 分子名称: RNA POLYMERASE ALPHA SUBUNIT (1 entity in total)
• 機能のキーワード: transcription regulation, nucleotidyl transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11094.72

構造登録者

Jeon, Y.H.,Negishi, T.,Shirakawa, M.,Yamazaki, T.,Fujita, N.,Ishihama, A.,Kyogoku, Y. (登録日: 1995-10-09, 公開日: 1996-03-08, 最終更新日: 2024-04-10)

主引用文献

Jeon, Y.H.,Negishi, T.,Shirakawa, M.,Yamazaki, T.,Fujita, N.,Ishihama, A.,Kyogoku, Y.
Solution structure of the activator contact domain of the RNA polymerase alpha subunit.
Science, 270:1495-1497, 1995

PubMed Abstract: The structure of the carboxyl-terminal domain of the Escherichia coli RNA polymerase alpha subunit (alpha CTD), which is regarded as the contact site for transcription activator proteins and for the promoter UP element, was determined by nuclear magnetic resonance spectroscopy. Its compact structure of four helices and two long arms enclosing its hydrophobic core shows a folding topology distinct from those of other DNA-binding proteins. The UP element binding site was found on the surface comprising helix 1, the amino-terminal end of helix 4, and the preceding loop. Mutation experiments indicated that the contact sites for transcription activator proteins are also on the same surface.

PubMed: 7491496

実験手法

SOLUTION NMR