1CMI

STRUCTURE OF THE HUMAN PIN/LC8 DIMER WITH A BOUND PEPTIDE

「1B1W」から置き換えられました

1CMI の概要

• エントリーDOI: 10.2210/pdb1cmi/pdb
• 分子名称: Dynein light chain 1, cytoplasmic, Nitric oxide synthase 1 (3 entities in total)
• 機能のキーワード: pin, lc8, nnos, dynein light chain, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 22737.97

構造登録者

Liang, J.,Guo, W.,Jaffery, S.,Snyder, S.,Clardy, J. (登録日: 1999-05-06, 公開日: 2000-02-21, 最終更新日: 2024-02-14)

主引用文献

Liang, J.,Jaffrey, S.R.,Guo, W.,Snyder, S.H.,Clardy, J.
Structure of the PIN/LC8 dimer with a bound peptide.
Nat.Struct.Biol., 6:735-740, 1999

PubMed Abstract: The structure of the protein known both as neuronal nitric oxide synthase inhibitory protein, PIN (protein inhibitor of nNOS), and also as the 8 kDa dynein light chain (LC8) has been solved by X-ray diffraction. Two PIN/LC8 monomers related by a two-fold axis form a rectangular dimer. Two pairs of alpha-helices cover opposite faces, and each pair of helices packs against a beta-sheet with five antiparallel beta-strands. Each five-stranded beta-sheet contains four strands from one monomer and a fifth strand from the other monomer. A 13-residue peptide from nNOS is bound to the dimer in a deep hydrophobic groove as a sixth antiparallel beta-strand. The structure provides key insights into dimerization of and peptide binding by the multifunctional PIN/LC8 protein.

PubMed: 10426949
DOI: 10.1038/11501

実験手法

X-RAY DIFFRACTION (2.5 Å)