1CMA

MET REPRESSOR/DNA COMPLEX + S-ADENOSYL-METHIONINE

Summary for 1CMA

• Entry DOI: 10.2210/pdb1cma/pdb
• Descriptor: DNA (5'-D(*TP*TP*AP*GP*AP*CP*GP*TP*CP*T)-3'), DNA (5'-D(*AP*GP*AP*CP*GP*TP*CP*TP*A)-3'), PROTEIN (MET REPRESSOR), ... (5 entities in total)
• Functional Keywords: protein-dna complex, double helix, transcription-dna complex, transcription/dna
• Biological source: Escherichia coli
• Cellular location: Cytoplasm: P0A8U6
• Total number of polymer chains: 4
• Total formula weight: 30626.81

Authors

Somers, W.S.,Phillips, S.E.V. (deposition date: 1992-08-24, release date: 1993-10-31, Last modification date: 2024-02-07)

Primary citation

Somers, W.S.,Phillips, S.E.
Crystal structure of the met repressor-operator complex at 2.8 A resolution reveals DNA recognition by beta-strands.
Nature, 359:387-393, 1992

PubMed Abstract: The crystal structure of the met repressor-operator complex shows two dimeric repressor molecules bound to adjacent sites 8 base pairs apart on an 18-base-pair DNA fragment. Sequence specificity is achieved by insertion of double-stranded antiparallel protein beta-ribbons into the major groove of B-form DNA, with direct hydrogen-bonding between amino-acid side chains and the base pairs. The repressor also recognizes sequence-dependent distortion or flexibility of the operator phosphate backbone, conferring specificity even for inaccessible base pairs.

PubMed: 1406951
DOI: 10.1038/359387a0

Experimental method

X-RAY DIFFRACTION (2.8 Å)