1CLD

DNA-binding protein

1CLD の概要

• エントリーDOI: 10.2210/pdb1cld/pdb
• 分子名称: CD2-LAC9, CADMIUM ION (2 entities in total)
• 機能のキーワード: zinc-binding domain, transcription regulation
• 由来する生物種: Kluyveromyces lactis
• 細胞内の位置: Nucleus: P08657
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7330.23

構造登録者

Gardner, K.H.,Coleman, J.E. (登録日: 1995-06-06, 公開日: 1995-09-15, 最終更新日: 2024-05-22)

主引用文献

Gardner, K.H.,Anderson, S.F.,Coleman, J.E.
Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.
Nat.Struct.Biol., 2:898-905, 1995

PubMed Abstract: The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

PubMed: 7552715
DOI: 10.1038/nsb1095-898

実験手法

SOLUTION NMR