1CKS

HUMAN CKSHS2 ATOMIC STRUCTURE: A ROLE FOR ITS HEXAMERIC ASSEMBLY IN CELL CYCLE CONTROL

1CKS の概要

• エントリーDOI: 10.2210/pdb1cks/pdb
• 分子名称: CYCLIN-DEPENDENT KINASE SUBUNIT, TYPE 2, SULFATE ION (3 entities in total)
• 機能のキーワード: cell division
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29926.28

構造登録者

Parge, H.E.,Arvai, A.S.,Tainer, J.A. (登録日: 1993-09-16, 公開日: 1995-02-07, 最終更新日: 2024-02-07)

主引用文献

Parge, H.E.,Arvai, A.S.,Murtari, D.J.,Reed, S.I.,Tainer, J.A.
Human CksHs2 atomic structure: a role for its hexameric assembly in cell cycle control.
Science, 262:387-395, 1993

PubMed Abstract: The cell cycle regulatory protein CksHs2 binds to the catalytic subunit of the cyclin-dependent kinases (Cdk's) and is essential for their biological function. The crystal structure of the protein was determined at 2.1 A resolution. The CksHs2 structure is an unexpected hexamer formed by the symmetric assembly of three interlocked dimers into an unusual 12-stranded beta barrel fold that may represent a prototype for this class of protein structures. Sequence-conserved regions form the unusual beta strand exchange between the subunits of the dimer, and the metal and anion binding sites associated with the hexamer assembly. The two other sequence-conserved regions line a 12 A diameter tunnel through the beta barrel and form the six exposed, charged helix pairs. Six kinase subunits can be modeled to bind the assembled hexamer without collision, and therefore this CksHs2 hexamer may participate in cell cycle control by acting as the hub for Cdk multimerization in vivo.

PubMed: 8211159

実験手法

X-RAY DIFFRACTION (2.1 Å)