1CJP
CONCANAVALIN A COMPLEX WITH 4'-METHYLUMBELLIFERYL-ALPHA-D-GLUCOPYRANOSIDE
Summary for 1CJP
Entry DOI | 10.2210/pdb1cjp/pdb |
Descriptor | CONCANAVALIN A, 4-METHYLUMBELLIFERYL-ALPHA-D-GLUCOSE, MANGANESE (II) ION, ... (5 entities in total) |
Functional Keywords | legume lectin, lectin |
Biological source | Canavalia ensiformis (jack bean) |
Total number of polymer chains | 4 |
Total formula weight | 104222.84 |
Authors | Hamodrakas, S.J.,Kanellopoulos, P.N.,Tucker, P.A. (deposition date: 1996-10-03, release date: 1997-10-15, Last modification date: 2024-05-22) |
Primary citation | Hamodrakas, S.J.,Kanellopoulos, P.N.,Pavlou, K.,Tucker, P.A. The crystal structure of the complex of concanavalin A with 4'-methylumbelliferyl-alpha-D-glucopyranoside. J.Struct.Biol., 118:23-30, 1997 Cited by PubMed Abstract: Concanavalin A (Con A) is the best known plant lectin, with important biological properties arising from its specific saccharide-binding ability. Its exact biological role still remains unknown. The complex of Con A with 4'-methylumbelliferyl-alpha-D-glucopyranoside (alpha-MUG) has been crystallized in space group P2(1) with cell dimensions a = 81.62 A, b = 128.71 A, c = 82.23 A, and beta = 118.47 degrees. X-ray diffraction intensities to 2.78 A have been collected. The structure of the complex was solved by molecular replacement and refined by simulated annealing methods to a crystallographic R-factor value of 0.182 and a free-R-factor value of 0.216. The asymmetric unit contains four subunits arranged as a tetramer, with approximate 222 symmetry. A saccharide molecule is bound in the sugar-binding site at the surface of each subunit, with the nonsugar (aglycon) part adopting a different orientation in each subunit. The aglycon orientation, although probably determined by packing of tetramers in the crystal lattice, helps to characterize the orientation of the saccharide in the sugar-binding pocket. The structure is the best determined alpha-D-glucoside:Con A complex to date and the hydrogen bonding network in the saccharide-binding site can be described with some confidence and compared with that of the alpha-D-mannosides. PubMed: 9087912DOI: 10.1006/jsbi.1996.3837 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.78 Å) |
Structure validation
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