1CJG

NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX

1CJG の概要

• エントリーDOI: 10.2210/pdb1cjg/pdb
• 分子名称: DNA (5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3'), PROTEIN (LAC REPRESSOR) (2 entities in total)
• 機能のキーワード: transcription regulation, lac operon, lac repressor, headpiece, lac operator, transcription-dna complex, transcription/dna
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 27140.24

構造登録者

Spronk, C.A.E.M.,Bonvin, A.M.J.J.,Radha, P.K.,Melacini, G.,Boelens, R.,Kaptein, R. (登録日: 1999-04-14, 公開日: 2000-01-01, 最終更新日: 2023-12-27)

主引用文献

Spronk, C.A.,Bonvin, A.M.,Radha, P.K.,Melacini, G.,Boelens, R.,Kaptein, R.
The solution structure of Lac repressor headpiece 62 complexed to a symmetrical lac operator.
Structure Fold.Des., 7:1483-1492, 1999

PubMed Abstract: Lactose repressor protein (Lac) controls the expression of the lactose metabolic genes in Escherichia coli by binding to an operator sequence in the promoter of the lac operon. Binding of inducer molecules to the Lac core domain induces changes in tertiary structure that are propagated to the DNA-binding domain through the connecting hinge region, thereby reducing the affinity for the operator. Protein-protein and protein-DNA interactions involving the hinge region play a crucial role in the allosteric changes occurring upon induction, but have not, as yet, been analyzed in atomic detail.

PubMed: 10647179
DOI: 10.1016/S0969-2126(00)88339-2

実験手法

SOLUTION NMR