1CHQ

SURPRISING LEADS FOR A CHOLERA TOXIN RECEPTOR BINDING ANTAGONIST; CRYSTALLOGRAPHIC STUDIES OF CTB MUTANTS

1CHQ の概要

• エントリーDOI: 10.2210/pdb1chq/pdb
• 分子名称: CHOLERA TOXIN B PENTAMER (2 entities in total)
• 機能のキーワード: toxin
• 由来する生物種: Vibrio cholerae
• 細胞内の位置: Secreted: P01556
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 57905.81

構造登録者

Merritt, E.A.,Hol, W.G.J. (登録日: 1995-02-15, 公開日: 1996-03-08, 最終更新日: 2024-11-13)

主引用文献

Merritt, E.A.,Sarfaty, S.,Chang, T.T.,Palmer, L.M.,Jobling, M.G.,Holmes, R.K.,Hol, W.G.
Surprising leads for a cholera toxin receptor-binding antagonist: crystallographic studies of CTB mutants.
Structure, 3:561-570, 1995

PubMed Abstract: Because agents which inhibit the receptor binding of cholera toxin constitute possible lead compounds for the structure-based design of anti-cholera drugs, detailed investigation of the toxin's receptor-binding site is of key importance. The substitution Gly-->Asp at residue 33 of the cholera toxin B subunit (CTB) has been reported to abolish receptor-binding ability. The substitution Arg35-->Asp has been reported to result in deficient assembly of the AB5 holotoxin. The molecular basis for these effects was not readily apparent from analysis of an earlier crystal structure of the wild-type toxin B pentamer in a complex with the receptor pentasaccharide.

PubMed: 8590017
DOI: 10.1016/S0969-2126(01)00190-3

実験手法

X-RAY DIFFRACTION (2.1 Å)