1CFW

GA-SUBSTITUTED DESULFOREDOXIN

1CFW の概要

• エントリーDOI: 10.2210/pdb1cfw/pdb
• 分子名称: PROTEIN (DESULFOREDOXIN), GALLIUM (III) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: rubredoxin type protein, electron transport
• 由来する生物種: Desulfovibrio gigas
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7850.21

構造登録者

Archer, M.,Carvalho, A.L.,Teixeira, S.,Moura, I.,Moura, J.J.G.,Rusnak, F.,Romao, M.J. (登録日: 1999-03-22, 公開日: 1999-07-09, 最終更新日: 2023-12-27)

主引用文献

Archer, M.,Carvalho, A.L.,Teixeira, S.,Moura, I.,Moura, J.J.,Rusnak, F.,Romao, M.J.
Structural studies by X-ray diffraction on metal substituted desulforedoxin, a rubredoxin-type protein.
Protein Sci., 8:1536-1545, 1999

PubMed Abstract: Desulforedoxin (Dx), isolated from the sulfate reducing bacterium Desulfovibrio gigas, is a small homodimeric (2 x 36 amino acids) protein. Each subunit contains a high-spin iron atom tetrahedrally bound to four cysteinyl sulfur atoms, a metal center similar to that found in rubredoxin (Rd) type proteins. The simplicity of the active center in Dx and the possibility of replacing the iron by other metals make this protein an attractive case for the crystallographic analysis of metal-substituted derivatives. This study extends the relevance of Dx to the bioinorganic chemistry field and is important to obtain model compounds that can mimic the four sulfur coordination of metals in biology. Metal replacement experiments were carried out by reconstituting the apoprotein with In3+, Ga3+, Cd2+, Hg2+, and Ni2+ salts. The In3+ and Ga3+ derivatives are isomorphous with the iron native protein; whereas Cd2+, Hg2+, and Ni2+ substituted Dx crystallized under different experimental conditions, yielding two additional crystal morphologies; their structures were determined by the molecular replacement method. A comparison of the three-dimensional structures for all metal derivatives shows that the overall secondary and tertiary structures are maintained, while some differences in metal coordination geometry occur, namely, bond lengths and angles of the metal with the sulfur ligands. These data are discussed in terms of the entatic state theory.

PubMed: 10422844

実験手法

X-RAY DIFFRACTION (1.9 Å)