1CDE

STRUCTURES OF APO AND COMPLEXED ESCHERICHIA COLI GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE

Summary for 1CDE

• Entry DOI: 10.2210/pdb1cde/pdb
• Related: 1CDD
• Descriptor: PHOSPHORIBOSYL-GLYCINAMIDE FORMYLTRANSFERASE, GLYCINAMIDE RIBONUCLEOTIDE, 5-DEAZAFOLIC ACID (3 entities in total)
• Functional Keywords: transferase(formyl)
• Biological source: Escherichia coli
• Total number of polymer chains: 4
• Total formula weight: 95963.29

Authors

Almassy, R.J.,Janson, C.A.,Kan, C.-C.,Hostomska, Z. (deposition date: 1992-05-15, release date: 1993-10-31, Last modification date: 2024-02-07)

Primary citation

Almassy, R.J.,Janson, C.A.,Kan, C.C.,Hostomska, Z.
Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.
Proc.Natl.Acad.Sci.USA, 89:6114-6118, 1992

PubMed Abstract: The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-A resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-A resolution. The structure is a modified doubly wound alpha/beta sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design.

PubMed: 1631098
DOI: 10.1073/pnas.89.13.6114

Experimental method

X-RAY DIFFRACTION (2.5 Å)