1C0C

BOVINE PANCREATIC RIBONUCLEASE A DESICCATED FOR 4.0 DAYS

1C0C の概要

• エントリーDOI: 10.2210/pdb1c0c/pdb
• 関連するPDBエントリー: 1BEL
• 分子名称: RIBONUCLEASE A, SULFATE ION (3 entities in total)
• 機能のキーワード: hydrolase (phosphoric diester, rna), desiccated, dry, dehydrated, hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14350.92

構造登録者

Bell, J.A. (登録日: 1999-07-15, 公開日: 1999-10-14, 最終更新日: 2024-10-30)

主引用文献

Bell, J.A.
X-ray crystal structures of a severely desiccated protein
Protein Sci., 8:2033-2040, 1999

PubMed Abstract: Unlike most protein crystals, form IX of bovine pancreatic ribonuclease A diffracts well when severely dehydrated. Crystal structures have been solved after 2.5 and 4 days of desiccation with CaSO4, at 1.9 and 2.0 A resolution, respectively. The two desiccated structures are very similar. An RMS displacement of 1.6 A is observed for main-chain atoms in each structure when compared to the hydrated crystal structure with some large rearrangements observed in loop regions. The structural changes are the result of intermolecular contacts formed by strong electrostatic interactions in the absence of a high dielectric medium. The electron density is very diffuse for some surface loops, consistent with a very disordered structure. This disorder is related to the conformational changes. These results help explain conformational changes during the lyophilization of protein and the associated phenomena of denaturation and molecular memory.

PubMed: 10548049

実験手法

X-RAY DIFFRACTION (2 Å)