1BYY

SODIUM CHANNEL IIA INACTIVATION GATE

1BYY の概要

• エントリーDOI: 10.2210/pdb1byy/pdb
• 分子名称: PROTEIN (SODIUM CHANNEL ALPHA-SUBUNIT) (1 entity in total)
• 機能のキーワード: sodium channel, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Membrane; Multi-pass membrane protein: P04775
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6266.27

構造登録者

Rohl, C.A.,Boeckman, F.A.,Baker, C.,Scheuer, T.,Catterall, W.A.,Klevit, R.E. (登録日: 1998-10-21, 公開日: 1999-10-29, 最終更新日: 2023-12-27)

主引用文献

Rohl, C.A.,Boeckman, F.A.,Baker, C.,Scheuer, T.,Catterall, W.A.,Klevit, R.E.
Solution structure of the sodium channel inactivation gate.
Biochemistry, 38:855-861, 1999

PubMed Abstract: The sodium channel initiates action potentials by opening in response to membrane depolarization. Fast channel inactivation, which is required for proper physiological function, is mediated by a cytoplasmic loop proposed to occlude the ion pore via a hinged lid mechanism with the triad IFM serving as a hydrophobic "latch". The NMR solution structure of the isolated inactivation gate reveals a stably folded core comprised of an alpha-helix capped by an N-terminal turn, supporting a model in which the tightly folded core containing the latch motif pivots on a more flexible hinge region to occlude the pore during inactivation. The structure, in combination with substituted cysteine mutagenesis experiments, indicates that the IFM triad and adjacent Thr are essential components of the latch and suggests differing roles for the residues of the IFMT motif in fast inactivation.

PubMed: 9893979
DOI: 10.1021/bi9823380

実験手法

SOLUTION NMR