1BYP

E43K,D44K DOUBLE MUTANT PLASTOCYANIN FROM SILENE

1BYP の概要

• エントリーDOI: 10.2210/pdb1byp/pdb
• 関連するPDBエントリー: 1BYO
• 分子名称: PROTEIN (PLASTOCYANIN), COPPER (II) ION (3 entities in total)
• 機能のキーワード: electron transfer, photosynthesis, acidic patch, double mutant, electron transport
• 由来する生物種: Silene latifolia subsp. alba
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P07030
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10479.21

構造登録者

Sugawara, H.,Inoue, T.,Li, C.,Gotowda, M.,Hibino, T.,Takabe, T.,Kai, Y. (登録日: 1998-10-19, 公開日: 1999-10-19, 最終更新日: 2023-08-09)

主引用文献

Sugawara, H.,Inoue, T.,Li, C.,Gotowda, M.,Hibino, T.,Takabe, T.,Kai, Y.
Crystal structures of wild-type and mutant plastocyanins from a higher plant, Silene.
J.Biochem.(Tokyo), 125:899-903, 1999

PubMed Abstract: Plastocyanin functions as an electron carrier between the cytochrome b6f complex and photosystem I. The crystal structures of the wild-type and E43K/D44K double mutant from the higher plant, Silene, have been determined at 2.0 and 1.75 A resolution, respectively. The wild-type plastocyanin comprises two monomers per asymmetric unit, one of which shows the unusually great distance between the copper ion and the Ndelta1 atom of H87 because of the hydrogen bond network formation between H87 and symmetry-related G10. The root mean square deviation for Ca atoms between the wild-type and mutant plastocyanins is 0.44 A, however, the electrostatic potential maps of their molecular surfaces are remarkably different. The low electron-transfer rate in the E43K/D44K mutant results from the hindrance of electrostatic interactions, not from the structural change due to the mutation.

PubMed: 10220581

実験手法

X-RAY DIFFRACTION (1.75 Å)