1BY5

FHUA FROM E. COLI, WITH ITS LIGAND FERRICHROME

1BY5 の概要

• エントリーDOI: 10.2210/pdb1by5/pdb
• 分子名称: FERRIC HYDROXAMATE UPTAKE PROTEIN, FERRICHROME, N-OCTYL-2-HYDROXYETHYL SULFOXIDE, ... (5 entities in total)
• 機能のキーワード: fhua, membrane protein, ligand-gated, iron transport, ferrichrome, metal binding protein
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cell outer membrane; Multi-pass membrane protein:
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81341.34

構造登録者

Locher, K.P.,Rees, B.,Koebnik, R.,Mitschler, A.,Moulinier, L.,Rosenbusch, J.P.,Moras, D. (登録日: 1998-10-23, 公開日: 1999-01-13, 最終更新日: 2023-11-15)

主引用文献

Locher, K.P.,Rees, B.,Koebnik, R.,Mitschler, A.,Moulinier, L.,Rosenbusch, J.P.,Moras, D.
Transmembrane signaling across the ligand-gated FhuA receptor: crystal structures of free and ferrichrome-bound states reveal allosteric changes.
Cell(Cambridge,Mass.), 95:771-778, 1998

PubMed Abstract: FhuA protein facilitates ligand-gated transport of ferrichrome-bound iron across Escherichia coli outer membranes. X-ray analysis at 2.7 A resolution reveals two distinct conformations in the presence and absence of ferrichrome. The monomeric protein consists of a hollow, 22-stranded, antiparallel beta barrel (residues 160-714), which is obstructed by a plug (residues 19-159). The binding site of ferrichrome, an aromatic pocket near the cell surface, undergoes minor changes upon association with the ligand. These are propagated and amplified across the plug, eventually resulting in substantially different protein conformations at the periplasmic face. Our findings reveal the mechanism of signal transmission and suggest how the energy-transducing TonB complex senses ligand binding.

PubMed: 9865695
DOI: 10.1016/S0092-8674(00)81700-6

実験手法

X-RAY DIFFRACTION (2.6 Å)