1BWI

THE 1.8 A STRUCTURE OF MICROBATCH OIL DROP GROWN TETRAGONAL HEN EGG WHITE LYSOZYME

1BWI の概要

• エントリーDOI: 10.2210/pdb1bwi/pdb
• 分子名称: PROTEIN (LYSOZYME) (2 entities in total)
• 機能のキーワード: lysozyme, 1, 4-beta-n-acetylmuramidase c, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• 細胞内の位置: Secreted: P00698
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14331.16

構造登録者

Dong, J.,Boggon, T.J.,Chayen, N.E.,Raftery, J.,Bi, R.C. (登録日: 1998-09-24, 公開日: 1998-09-30, 最終更新日: 2024-10-09)

主引用文献

Dong, J.,Boggon, T.J.,Chayen, N.E.,Raftery, J.,Bi, R.C.,Helliwell, J.R.
Bound-solvent structures for microgravity-, ground control-, gel- and microbatch-grown hen egg-white lysozyme crystals at 1.8 A resolution.
Acta Crystallogr.,Sect.D, 55:745-752, 1999

PubMed Abstract: A number of methods can be used to improve the stability of the protein crystal-growth environment, including growth in microgravity without an air-liquid phase boundary, growth in gels and growth under oil ('microbatch'). In this study, X-ray data has been collected from and structures refined for crystals of hen egg-white lysozyme (HEWL) grown using four different methods, liquid-liquid dialysis on Earth and in microgravity using the European Space Agency's (ESA) Advanced Protein Crystallization Facility (APCF) on board the NASA Space Shuttle Life and Microgravity Spacelab (LMS) mission (STS-78), crystallization in agarose gel using a tube liquid-gel diffusion method and crystallization in microbatch under oil. A comparison of the overall quality of the X-ray data, the protein structures and especially the bound-water structures has been carried out at 1.8 A. The lysozyme protein structures corresponding to these four different crystallization methods remain similar. A small improvement in the bound-solvent structure is seen in lysozyme crystals grown in microgravity by liquid-liquid dialysis, which has a more stable fluid physics state in microgravity, and is consistent with a better formed protein crystal in microgravity.

PubMed: 10089304
DOI: 10.1107/S0907444998016047

実験手法

X-RAY DIFFRACTION (1.8 Å)