1BSO

12-BROMODODECANOIC ACID BINDS INSIDE THE CALYX OF BOVINE BETA-LACTOGLOBULIN

1BSO の概要

• エントリーDOI: 10.2210/pdb1bso/pdb
• 分子名称: PROTEIN (BOVINE BETA-LACTOGLOBULIN A), 12-BROMODODECANOIC ACID (3 entities in total)
• 機能のキーワード: beta-lactoglobulin, ligand binding, x-ray crystal structure, transport protein
• 由来する生物種: Bos taurus (cattle)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18666.48

構造登録者

Qin, B.Y.,Creamer, L.K.,Baker, E.N.,Jameson, G.B. (登録日: 1998-08-29, 公開日: 1999-09-02, 最終更新日: 2024-10-30)

主引用文献

Qin, B.Y.,Creamer, L.K.,Baker, E.N.,Jameson, G.B.
12-Bromododecanoic acid binds inside the calyx of bovine beta-lactoglobulin.
FEBS Lett., 438:272-278, 1998

PubMed Abstract: The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carboxylate head group lies at the surface of the molecule, and the lid to the calyx is open at the pH of crystallization (pH 7.3), consistent with the conformation observed in ligand-free bovine beta-lactoglobulin in lattice Z at pH 7.1 and pH 8.2.

PubMed: 9827560
DOI: 10.1016/S0014-5793(98)01199-5

実験手法

X-RAY DIFFRACTION (2.23 Å)