1BRW

THE CRYSTAL STRUCTURE OF PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE IN A CLOSED CONFORMATION

1BRW の概要

• エントリーDOI: 10.2210/pdb1brw/pdb
• 分子名称: PROTEIN (PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE), PHOSPHATE ION, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: nucleoside phosphorylase, domain movement, transferase
• 由来する生物種: Geobacillus stearothermophilus
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92899.03

構造登録者

Pugmire, M.J.,Ealick, S.E. (登録日: 1998-08-25, 公開日: 1999-01-13, 最終更新日: 2023-08-09)

主引用文献

Pugmire, M.J.,Ealick, S.E.
The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
Structure, 6:1467-1479, 1998

PubMed Abstract: Pyrimidine nucleoside phosphorylase (PYNP) catalyzes the reversible phosphorolysis of pyrimidines in the nucleotide synthesis salvage pathway. In lower organisms (e.g. Bacillus stearothermophilus) PYNP accepts both thymidine and uridine, whereas in mammalian and other higher organisms it is specific for thymidine (designated thymidine phosphorylase, TP). PYNP shares 40% sequence similarity (and presumably significant structural similarity) with human TP, which has been implicated as a growth factor in tumor angiogenesis. It is thought that TP undergoes a major conformational change upon substrate binding that consequently produces an active conformation.

PubMed: 9817849
DOI: 10.1016/S0969-2126(98)00145-2

実験手法

X-RAY DIFFRACTION (2.1 Å)