1BR3

CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME

Summary for 1BR3

• Entry DOI: 10.2210/pdb1br3/pdb
• Descriptor: RNA (5'-R(*GP*GP*AP*CP*AP*GP*AP*UP*GP*GP*GP*AP*G)-3'), DNA (10-23 DNA ENZYME) (2 entities in total)
• Functional Keywords: dna enzyme, ribozyme, holliday junction, dna/rna hybrid, dna-rna hybrid complex, dna-rna hybrid
• Total number of polymer chains: 2
• Total formula weight: 12811.14

Authors

Nowakowski, J.,Shim, P.J.,Prasad, G.S.,Stout, C.D.,Joyce, G.F. (deposition date: 1998-08-13, release date: 1999-02-23, Last modification date: 2024-02-07)

Primary citation

Nowakowski, J.,Shim, P.J.,Prasad, G.S.,Stout, C.D.,Joyce, G.F.
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme.
Nat.Struct.Biol., 6:151-156, 1999

PubMed Abstract: The structure of a large nucleic acid complex formed by the 10-23 DNA enzyme bound to an RNA substrate was determined by X-ray diffraction at 3.0 A resolution. The 82-nucleotide complex contains two strands of DNA and two strands of RNA that form five double-helical domains. The spatial arrangement of these helices is maintained by two four-way junctions that exhibit extensive base-stacking interactions and sharp turns of the phosphodiester backbone stabilized by metal ions coordinated to nucleotides at these junctions. Although it is unlikely that the structure corresponds to the catalytically active conformation of the enzyme, it represents a novel nucleic acid fold with implications for the Holliday junction structure.

PubMed: 10048927
DOI: 10.1038/5839

Experimental method

X-RAY DIFFRACTION (3 Å)