1BR3

CRYSTAL STRUCTURE OF AN 82-NUCLEOTIDE RNA-DNA COMPLEX FORMED BY THE 10-23 DNA ENZYME

1BR3 の概要

• エントリーDOI: 10.2210/pdb1br3/pdb
• 分子名称: RNA (5'-R(*GP*GP*AP*CP*AP*GP*AP*UP*GP*GP*GP*AP*G)-3'), DNA (10-23 DNA ENZYME) (2 entities in total)
• 機能のキーワード: dna enzyme, ribozyme, holliday junction, dna/rna hybrid, dna-rna hybrid complex, dna-rna hybrid
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 12811.14

構造登録者

Nowakowski, J.,Shim, P.J.,Prasad, G.S.,Stout, C.D.,Joyce, G.F. (登録日: 1998-08-13, 公開日: 1999-02-23, 最終更新日: 2024-02-07)

主引用文献

Nowakowski, J.,Shim, P.J.,Prasad, G.S.,Stout, C.D.,Joyce, G.F.
Crystal structure of an 82-nucleotide RNA-DNA complex formed by the 10-23 DNA enzyme.
Nat.Struct.Biol., 6:151-156, 1999

PubMed Abstract: The structure of a large nucleic acid complex formed by the 10-23 DNA enzyme bound to an RNA substrate was determined by X-ray diffraction at 3.0 A resolution. The 82-nucleotide complex contains two strands of DNA and two strands of RNA that form five double-helical domains. The spatial arrangement of these helices is maintained by two four-way junctions that exhibit extensive base-stacking interactions and sharp turns of the phosphodiester backbone stabilized by metal ions coordinated to nucleotides at these junctions. Although it is unlikely that the structure corresponds to the catalytically active conformation of the enzyme, it represents a novel nucleic acid fold with implications for the Holliday junction structure.

PubMed: 10048927
DOI: 10.1038/5839

実験手法

X-RAY DIFFRACTION (3 Å)