1BQ1

E. COLI THYMIDYLATE SYNTHASE MUTANT N177A IN COMPLEX WITH CB3717 AND 2'-DEOXYURIDINE 5'-MONOPHOSPHATE (DUMP)

Summary for 1BQ1

• Entry DOI: 10.2210/pdb1bq1/pdb
• Descriptor: THYMIDYLATE SYNTHASE, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, 10-PROPARGYL-5,8-DIDEAZAFOLIC ACID, ... (4 entities in total)
• Functional Keywords: transferase, active site mutant, reaction intermediate
• Biological source: Escherichia coli
• Cellular location: Cytoplasm : P0A884
• Total number of polymer chains: 2
• Total formula weight: 62604.59

Authors

Reyes, C.L.,Sage, C.R.,Rutenber, E.E.,Finer-Moore, J.S.,Stroud, R.M. (deposition date: 1998-08-20, release date: 1999-05-18, Last modification date: 2024-10-30)

Primary citation

Reyes, C.L.,Sage, C.R.,Rutenber, E.E.,Nissen, R.M.,Finer-Moore, J.S.,Stroud, R.M.
Inactivity of N229A thymidylate synthase due to water-mediated effects: isolating a late stage in methyl transfer.
J.Mol.Biol., 284:699-712, 1998

PubMed Abstract: Mutation of thymidylate synthase N229(177) to alanine results in an essentially inactive enzyme, yet it leads to formation of a stable ternary complex. The kinetics of N229(177)A show that kcat for Escherichia coli is reduced by 200-fold while the Km for dUMP is increased 200-fold and the Km for folate increased by tenfold versus the wild-type enzyme. The crystal structures of N229(177)A in complex with dUMP and CB3717, and in complex with dUMP alone are determined at 2.4 A, and 2.5 A resolution. These structures identify the covalently bound ternary complex and show how N229(177)A traps an intermediate, and so becomes inactive in a later step of the reaction. Since the smaller alanine side-chain at N229(177)A does not directly sterically impair binding of ligands, the structures implicate, and place quantitative limits on the involvement of the structured water network in the active site of thymidylate synthase in both catalysis and in determining the binding affinity for dUMP (in contrast, the N229(177)V mutation in Lactobacillus casei has minimal effect on activity).

PubMed: 9826509
DOI: 10.1006/jmbi.1998.2205

Experimental method

X-RAY DIFFRACTION (2.5 Å)