1BNZ

SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX

Summary for 1BNZ

• Entry DOI: 10.2210/pdb1bnz/pdb
• NMR Information: BMRB: 5909,5910
• Descriptor: 5'-D(*GP*TP*AP*AP*TP*TP*AP*C)-3', DNA-BINDING PROTEIN 7A (3 entities in total)
• Functional Keywords: protein-dna interaction, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Sulfolobus acidocaldarius
• Total number of polymer chains: 3
• Total formula weight: 12146.84

Authors

Gao, Y.-G.,Su, S.-Y.,Robinson, H.,Padmanabhan, S.,Lim, L.,Mccrary, B.S.,Edmondos, S.P.,Shrive, J.W.,Wang, A.H.-J. (deposition date: 1998-07-31, release date: 1998-11-11, Last modification date: 2024-02-07)

Primary citation

Gao, Y.G.,Su, S.Y.,Robinson, H.,Padmanabhan, S.,Lim, L.,McCrary, B.S.,Edmondson, S.P.,Shriver, J.W.,Wang, A.H.
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA.
Nat.Struct.Biol., 5:782-786, 1998

PubMed Abstract: Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.

PubMed: 9731772
DOI: 10.1038/1822

Experimental method

X-RAY DIFFRACTION (2 Å)