1BNZ

SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX

1BNZ の概要

• エントリーDOI: 10.2210/pdb1bnz/pdb
• NMR情報: BMRB: 5909,5910
• 分子名称: 5'-D(*GP*TP*AP*AP*TP*TP*AP*C)-3', DNA-BINDING PROTEIN 7A (3 entities in total)
• 機能のキーワード: protein-dna interaction, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Sulfolobus acidocaldarius
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 12146.84

構造登録者

Gao, Y.-G.,Su, S.-Y.,Robinson, H.,Padmanabhan, S.,Lim, L.,Mccrary, B.S.,Edmondos, S.P.,Shrive, J.W.,Wang, A.H.-J. (登録日: 1998-07-31, 公開日: 1998-11-11, 最終更新日: 2024-02-07)

主引用文献

Gao, Y.G.,Su, S.Y.,Robinson, H.,Padmanabhan, S.,Lim, L.,McCrary, B.S.,Edmondson, S.P.,Shriver, J.W.,Wang, A.H.
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA.
Nat.Struct.Biol., 5:782-786, 1998

PubMed Abstract: Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus solfataricus and S. acidocaldarius respectively. These proteins have high thermal, acid and chemical stability. They bind DNA without marked sequence preference and increase the Tm of DNA by approximately 40 degrees C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was crystallized in different crystal lattices and the crystal structures were solved at high resolution. Sso7d binds in the minor groove of DNA and causes a single-step sharp kink in DNA (approximately 60 degrees) by the intercalation of the hydrophobic side chains of Val 26 and Met 29. The intercalation sites are different in the two complexes. Observations of this novel DNA binding mode in three independent crystal lattices indicate that it is not a function of crystal packing.

PubMed: 9731772
DOI: 10.1038/1822

実験手法

X-RAY DIFFRACTION (2 Å)