1BN9

RESPONSE ELEMENT OF THE ORPHAN NUCLEAR RECEPTOR REV-ERB BETA

Summary for 1BN9

• Entry DOI: 10.2210/pdb1bn9/pdb
• NMR Information: BMRB: 4172
• Descriptor: DNA (5'-D(*TP*AP*GP*AP*AP*TP*GP*TP*AP*GP*GP*TP*CP*AP*G)-3'), DNA (5'-D(*CP*TP*GP*AP*CP*CP*TP*AP*CP*AP*TP*TP*CP*TP*A)-3') (2 entities in total)
• Functional Keywords: dna, response element, orphan nuclear receptor
• Total number of polymer chains: 2
• Total formula weight: 9177.01

Authors

Castagne, C.,Terenzi, H.,Zakin, M.M.,Delepierre, M. (deposition date: 1998-07-31, release date: 1998-08-05, Last modification date: 2023-12-27)

Primary citation

Castagne, C.,Terenzi, H.,Zakin, M.M.,Delepierre, M.
Solution structure of the orphan nuclear receptor rev-erb beta response element by 1H, 31P NMR and molecular simulation
Biochimie, 82:739-748, 2000

PubMed Abstract: Rev-erb beta is an orphan receptor that binds as a homodimer or as a monomer to DNA. The solution structure of the non-palindromic 15 bp DNA duplex d(TAGAATGTAGGTCAG), the response element of Rev-erb beta for monomeric binding, was determined by 1H and 31P NMR, energy minimization with NMR-derived restraints for distances and NOE back-calculation methods. The refined final structures have the typical overall features of B-type DNA. However, titration of this 15 bp duplex with ReDBD, the DNA binding domain of Rev-erb beta, showed large shifts of imino protons and 31P signals, suggesting major conformational changes.

PubMed: 11018291
DOI: 10.1016/S0300-9084(00)01148-2

Experimental method

SOLUTION NMR