1BM1

CRYSTAL STRUCTURE OF BACTERIORHODOPSIN IN THE LIGHT-ADAPTED STATE

1BM1 の概要

• エントリーDOI: 10.2210/pdb1bm1/pdb
• 分子名称: BACTERIORHODOPSIN, RETINAL, PHOSPHORIC ACID 2,3-BIS-(3,7,11,15-TETRAMETHYL-HEXADECYLOXY)-PROPYL ESTER 2-HYDROXO-3-PHOSPHONOOXY-PROPYL ESTER (3 entities in total)
• 機能のキーワード: proton pump, membrane protein, retinal protein, photoreceptor
• 由来する生物種: Halobacterium salinarum
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P02945
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27969.01

構造登録者

Sato, H.,Takeda, K.,Tani, K.,Hino, T.,Okada, T.,Nakasako, M.,Kamiya, N.,Kouyama, T. (登録日: 1998-07-28, 公開日: 1999-04-27, 最終更新日: 2024-11-13)

主引用文献

Sato, H.,Takeda, K.,Tani, K.,Hino, T.,Okada, T.,Nakasako, M.,Kamiya, N.,Kouyama, T.
Specific lipid-protein interactions in a novel honeycomb lattice structure of bacteriorhodopsin.
Acta Crystallogr.,Sect.D, 55:1251-1256, 1999

PubMed Abstract: In the purple membrane of Halobacterium salinarium, bacteriorhodopsin trimers are arranged in a hexagonal lattice. When purple membrane sheets are incubated at high temperature with neutral detergent, membrane vesicularization takes place, yielding inside-out vesicles with a diameter of 50 nm. The vesicular structure becomes unstable at low temperature, where successive fusion of the vesicles yields a crystal which is composed of stacked planar membranes. X-ray crystallographic analysis reveals that the bacteriorhodopsin trimers are arranged in a honeycomb lattice in each membrane layer and that neighbouring membranes orient in opposite directions. The native structure of the trimeric unit is preserved in the honeycomb lattice, irrespective of alterations in the in-plane orientation of the trimer. One phospholipid tightly bound to a crevice between monomers in the trimeric unit is suggested to act as a glue in the formation of the trimer.

PubMed: 10393291
DOI: 10.1107/S090744499900503X

実験手法

X-RAY DIFFRACTION (3.5 Å)