1BLI

BACILLUS LICHENIFORMIS ALPHA-AMYLASE

1BLI の概要

• エントリーDOI: 10.2210/pdb1bli/pdb
• 分子名称: ALPHA-AMYLASE, CALCIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: hydrolase, glycosyltransferase, alpha-amylase, starch degradation, alpha-1, 4-glucan-4-glucanohydrolase, thermostability, calcium, sodium
• 由来する生物種: Bacillus licheniformis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55410.18

構造登録者

Machius, M.,Declerck, N.,Huber, R.,Wiegand, G. (登録日: 1998-01-07, 公開日: 1999-03-23, 最終更新日: 2024-05-22)

主引用文献

Machius, M.,Declerck, N.,Huber, R.,Wiegand, G.
Activation of Bacillus licheniformis alpha-amylase through a disorder-->order transition of the substrate-binding site mediated by a calcium-sodium-calcium metal triad.
Structure, 6:281-292, 1998

PubMed Abstract: The structural basis as to how metals regulate the functional state of a protein by altering or stabilizing its conformation has been characterized in relatively few cases because the metal-free form of the protein is often partially disordered and unsuitable for crystallographic analysis. This is not the case, however, for Bacillus licheniformis alpha-amylase (BLA) for which the structure of the metal-free form is available. BLA is a hyperthermostable enzyme which is widely used in biotechnology, for example in the breakdown of starch or as a component of detergents. The determination of the structure of BLA in the metal-containing form, together with comparisons to the apo enzyme, will help us to understand the way in which metal ions can regulate enzyme activity.

PubMed: 9551551
DOI: 10.1016/S0969-2126(98)00032-X

実験手法

X-RAY DIFFRACTION (1.9 Å)