1BKD

COMPLEX OF HUMAN H-RAS WITH HUMAN SOS-1

1BKD の概要

• エントリーDOI: 10.2210/pdb1bkd/pdb
• 分子名称: H-RAS, SON OF SEVENLESS-1 (3 entities in total)
• 機能のキーワード: complex (oncogene protein-exchange factor), small gtpase, exchange factor, complex(oncogene protein-exchange factor) complex, complex(oncogene protein/exchange factor)
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor; Cytoplasmic side: P01112
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 74894.29

構造登録者

Boriack-Sjodin, P.A.,Margarit, S.M.,Sagi, D.B.,Kuriyan, J. (登録日: 1998-07-06, 公開日: 1999-01-13, 最終更新日: 2024-02-07)

主引用文献

Boriack-Sjodin, P.A.,Margarit, S.M.,Bar-Sagi, D.,Kuriyan, J.
The structural basis of the activation of Ras by Sos.
Nature, 394:337-343, 1998

PubMed Abstract: The crystal structure of human H-Ras complexed with the Ras guanine-nucleotide-exchange-factor region of the Son of sevenless (Sos) protein has been determined at 2.8 A resolution. The normally tight interaction of nucleotides with Ras is disrupted by Sos in two ways. First, the insertion into Ras of an alpha-helix from Sos results in the displacement of the Switch 1 region of Ras, opening up the nucleotide-binding site. Second, side chains presented by this helix and by a distorted conformation of the Switch 2 region of Ras alter the chemical environment of the binding site for the phosphate groups of the nucleotide and the associated magnesium ion, so that their binding is no longer favoured. Sos does not impede the binding sites for the base and the ribose of GTP or GDP, so the Ras-Sos complex adopts a structure that allows nucleotide release and rebinding.

PubMed: 9690470
DOI: 10.1038/28548

実験手法

X-RAY DIFFRACTION (2.8 Å)