1BK5

KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE

1BK5 の概要

• エントリーDOI: 10.2210/pdb1bk5/pdb
• 分子名称: KARYOPHERIN ALPHA, COBALT (II) ION (3 entities in total)
• 機能のキーワード: protein transport, nls nuclear import, armadillo repeat containing protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 93554.72

構造登録者

Conti, E.,Uy, M.,Leighton, L.,Blobel, G.,Kuriyan, J. (登録日: 1998-07-14, 公開日: 1999-01-06, 最終更新日: 2024-02-07)

主引用文献

Conti, E.,Uy, M.,Leighton, L.,Blobel, G.,Kuriyan, J.
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha.
Cell(Cambridge,Mass.), 94:193-204, 1998

PubMed Abstract: Selective nuclear import is mediated by nuclear localization signals (NLSs) and cognate transport factors known as karyopherins or importins. Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs. We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast karyopherin alpha, in the absence and presence of a monopartite NLS peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a tandem array of ten armadillo repeats, organized in a right-handed superhelix of helices. Binding of the NLS peptide occurs at two sites within a helical surface groove that is lined by conserved residues. The structure reveals the determinants of NLS specificity and suggests a model for the recognition of bipartite NLSs.

PubMed: 9695948
DOI: 10.1016/S0092-8674(00)81419-1

実験手法

X-RAY DIFFRACTION (2.2 Å)