1BIX

THE CRYSTAL STRUCTURE OF THE HUMAN DNA REPAIR ENDONUCLEASE HAP1 SUGGESTS THE RECOGNITION OF EXTRA-HELICAL DEOXYRIBOSE AT DNA ABASIC SITES

1BIX の概要

• エントリーDOI: 10.2210/pdb1bix/pdb
• 分子名称: AP ENDONUCLEASE 1, SAMARIUM (III) ION, PLATINUM (II) ION, ... (4 entities in total)
• 機能のキーワード: dna repair, endonuclease, hap1, ref-1, abasic site recognition
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus. DNA-(apurinic or apyrimidinic site) lyase, mitochondrial: Mitochondrion: P27695
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33084.20

構造登録者

Gorman, M.A.,Morera, S.,Rothwell, D.G.,De La Fortelle, E.,Mol, C.D.,Tainer, J.A.,Hickson, I.D.,Freemont, P.S. (登録日: 1998-06-19, 公開日: 1999-06-22, 最終更新日: 2024-02-07)

主引用文献

Gorman, M.A.,Morera, S.,Rothwell, D.G.,de La Fortelle, E.,Mol, C.D.,Tainer, J.A.,Hickson, I.D.,Freemont, P.S.
The crystal structure of the human DNA repair endonuclease HAP1 suggests the recognition of extra-helical deoxyribose at DNA abasic sites.
EMBO J., 16:6548-6558, 1997

PubMed Abstract: The structure of the major human apurinic/ apyrimidinic endonuclease (HAP1) has been solved at 2.2 A resolution. The enzyme consists of two symmetrically related domains of similar topology and has significant structural similarity to both bovine DNase I and its Escherichia coli homologue exonuclease III (EXOIII). A structural comparison of these enzymes reveals three loop regions specific to HAP1 and EXOIII. These loop regions apparently act in DNA abasic site (AP) recognition and cleavage since DNase I, which lacks these loops, correspondingly lacks AP site specificity. The HAP1 structure furthermore suggests a mechanism for AP site binding which involves the recognition of the deoxyribose moiety in an extrahelical conformation, rather than a 'flipped-out' base opposite the AP site.

PubMed: 9351835
DOI: 10.1093/emboj/16.21.6548

実験手法

X-RAY DIFFRACTION (2.2 Å)