1BG7

LOCALIZED UNFOLDING AT THE JUNCTION OF THREE FERRITIN SUBUNITS. A MECHANISM FOR IRON RELEASE?

Summary for 1BG7

Entry DOI	10.2210/pdb1bg7/pdb
Descriptor	FERRITIN, CALCIUM ION (3 entities in total)
Functional Keywords	ferritin, iron storage
Biological source	Rana catesbeiana (bullfrog)
Total number of polymer chains	1
Total formula weight	20586.18
Authors	Takagi, H.,Shi, D.,Ha, Y.,Allewell, N.M.,Theil, E.C. (deposition date: 1998-06-05, release date: 1999-01-13, Last modification date: 2024-05-22)
Primary citation	Takagi, H.,Shi, D.,Ha, Y.,Allewell, N.M.,Theil, E.C. Localized unfolding at the junction of three ferritin subunits. A mechanism for iron release? J.Biol.Chem., 273:18685-18688, 1998 Cited by PubMed Abstract: How and where iron exits from ferritin for cellular use is unknown. Twenty-four protein subunits create a cavity in ferritin where iron is concentrated >10(11)-fold as a mineral. Proline substitution for conserved leucine 134 (L134P) allowed normal assembly but increased iron exit rates. X-ray crystallography of H-L134P ferritin revealed localized unfolding at the 3-fold axis, also iron entry sites, consistent with shared use sites for iron exit and entry. The junction of three ferritin subunits appears to be a dynamic aperture with a "shutter" that cytoplasmic factors might open or close to regulate iron release in vivo. PubMed: 9668036 DOI: 10.1074/jbc.273.30.18685 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.85 Å)

Structure validation