1BFE

THE THIRD PDZ DOMAIN FROM THE SYNAPTIC PROTEIN PSD-95

1BFE の概要

• エントリーDOI: 10.2210/pdb1bfe/pdb
• 分子名称: PSD-95 (2 entities in total)
• 機能のキーワード: peptide recognition, protein localization
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cell membrane ; Lipid-anchor ; Cytoplasmic side : P31016
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12750.13

構造登録者

Doyle, D.A.,Lee, A.,Lewis, J.,Kim, E.,Sheng, M.,Mackinnon, R. (登録日: 1998-05-20, 公開日: 1998-10-21, 最終更新日: 2024-02-07)

主引用文献

Doyle, D.A.,Lee, A.,Lewis, J.,Kim, E.,Sheng, M.,MacKinnon, R.
Crystal structures of a complexed and peptide-free membrane protein-binding domain: molecular basis of peptide recognition by PDZ.
Cell(Cambridge,Mass.), 85:1067-1076, 1996

PubMed Abstract: Modular PDZ domains, found in many cell junction-associated proteins, mediate the clustering of membrane ion channels by binding to their C-terminus. The X-ray crystallographic structures of the third PDZ domain from the synaptic protein PSD-95 in complex with and in the absence of its peptide ligand have been determined at 1.8 angstroms and 2.3 angstroms resolution, respectively. The structures reveal that a four-residue C-terminal stretch (X-Thr/Ser-X-Val-COO(-)) engages the PDZ domain through antiparallel main chain interactions with a beta sheet of the domain. Recognition of the terminal carboxylate group of the peptide is conferred by a cradle of main chain amides provided by a Gly-Leu-Gly-Phe loop as well as by an arginine side chain. Specific side chain interactions and a prominent hydrophobic pocket explain the selective recognition of the C-terminal consensus sequence.

PubMed: 8674113
DOI: 10.1016/S0092-8674(00)81307-0

実験手法

X-RAY DIFFRACTION (2.3 Å)