1BDV

ARC FV10 COCRYSTAL

1BDV の概要

• エントリーDOI: 10.2210/pdb1bdv/pdb
• 分子名称: DNA (5'-D(*TP*AP*TP*AP*GP*TP*AP*GP*AP*GP*TP*GP*CP*TP*TP*CP*TP*AP*TP*CP*AP*T)-3'), DNA (5'-D(*AP*AP*TP*GP*AP*TP*AP*GP*AP*AP*GP*CP*AP*CP*TP*CP*TP*AP*CP*TP*AP*T)-3'), PROTEIN (ARC FV10 REPRESSOR), ... (4 entities in total)
• 機能のキーワード: gene-regulating protein, gene regulation-dna complex, gene regulation/dna
• 由来する生物種: Enterobacteria phage P22
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 38260.71

構造登録者

Schildbach, J.F.,Karzai, A.W.,Raumann, B.E.,Sauer, R.T. (登録日: 1998-05-11, 公開日: 1999-01-06, 最終更新日: 2023-08-02)

主引用文献

Schildbach, J.F.,Karzai, A.W.,Raumann, B.E.,Sauer, R.T.
Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.
Proc.Natl.Acad.Sci.USA, 96:811-817, 1999

PubMed Abstract: A central question in protein-DNA recognition is the origin of the specificity that permits binding to the correct site in the presence of excess, nonspecific DNA. In the P22 Arc repressor, the Phe-10 side chain is part of the hydrophobic core of the free protein but rotates out to pack against the sugar-phosphate backbone of the DNA in the repressor-operator complex. Characterization of a library of position 10 variants reveals that Phe is the only residue that results in fully active Arc. One class of mutants folds stably but binds operator with reduced affinity; another class is unstable. FV10, one member of the first class, binds operator DNA and nonoperator DNA almost equally well. The affinity differences between FV10 and wild type indicate that each Phe-10 side chain contributes 1.5-2.0 kcal to operator binding but less than 0.5 kcal/mol to nonoperator binding, demonstrating that contacts between Phe-10 and the operator DNA backbone contribute to binding specificity. This appears to be a direct contribution as the crystal structure of the FV10 dimer is similar to wild type and the Phe-10-DNA backbone interactions are the only contacts perturbed in the cocrystal structure of the FV10-operator complex.

PubMed: 9927650
DOI: 10.1073/pnas.96.3.811

実験手法

X-RAY DIFFRACTION (2.8 Å)