1B7F

SXL-LETHAL PROTEIN/RNA COMPLEX

1B7F の概要

• エントリーDOI: 10.2210/pdb1b7f/pdb
• 分子名称: RNA (5'-R(P*GP*UP*UP*GP*UP*UP*UP*UP*UP*UP*UP*U)-3'), PROTEIN (SXL-LETHAL PROTEIN) (3 entities in total)
• 機能のキーワード: splicing regulation, rnp domain, rna complex, riken structural genomics/proteomics initiative, rsgi, structural genomics, rna-binding protein-rna complex, rna-binding protein/rna
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 45357.38

構造登録者

Handa, N.,Nureki, O.,Kurimoto, K.,Kim, I.,Sakamoto, H.,Shimura, Y.,Muto, Y.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 1999-01-23, 公開日: 1999-05-03, 最終更新日: 2023-12-27)

主引用文献

Handa, N.,Nureki, O.,Kurimoto, K.,Kim, I.,Sakamoto, H.,Shimura, Y.,Muto, Y.,Yokoyama, S.
Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein.
Nature, 398:579-585, 1999

PubMed Abstract: The Sex-lethal (Sxl) protein of Drosophila melanogaster regulates alternative splicing of the transformer (tra) messenger RNA precursor by binding to the tra polypyrimidine tract during the sex-determination process. The crystal structure has now been determined at 2.6 A resolution of the complex formed between two tandemly arranged RNA-binding domains of the Sxl protein and a 12-nucleotide, single-stranded RNA derived from the tra polypyrimidine tract. The two RNA-binding domains have their beta-sheet platforms facing each other to form a V-shaped cleft. The RNA is characteristically extended and bound in this cleft, where the UGUUUUUUU sequence is specifically recognized by the protein. This structure offers the first insight, to our knowledge, into how a protein binds specifically to a cognate RNA without any intramolecular base-pairing.

PubMed: 10217141
DOI: 10.1038/19242

実験手法

X-RAY DIFFRACTION (2.6 Å)