1B68

APOLIPOPROTEIN E4 (APOE4), 22K FRAGMENT

1B68 の概要

• エントリーDOI: 10.2210/pdb1b68/pdb
• 分子名称: APOLIPOPROTEIN E (2 entities in total)
• 機能のキーワード: lipid transport, heparin-binding, plasma protein, hdl, vldl
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22216.13

構造登録者

Rupp, B.,Peters-Libeu, C. (登録日: 1999-01-21, 公開日: 2001-07-11, 最終更新日: 2023-08-09)

主引用文献

Dong, J.,Peters-Libeu, C.A.,Weisgraber, K.H.,Segelke, B.W.,Rupp, B.,Capila, I.,Hernaiz, M.J.,LeBrun, L.A.,Linhardt, R.J.
Interaction of the N-terminal domain of apolipoprotein E4 with heparin.
Biochemistry, 40:2826-2834, 2001

PubMed Abstract: Apolipoprotein E (apoE) is an important lipid-transport protein in human plasma and brain. It has three common isoforms (apoE2, apoE3, and apoE4). ApoE is a major genetic risk factor in heart disease and in neurodegenerative disease, including Alzheimer's disease. The interaction of apoE with heparan sulfate proteoglycans plays an important role in lipoprotein remnant uptake and likely in atherogenesis and Alzheimer's disease. Here we report our studies of the interaction of the N-terminal domain of apoE4 (residues 1-191), which contains the major heparin-binding site, with an enzymatically prepared heparin oligosaccharide. Identified by its high affinity for the N-terminal domain of apoE4, this oligosaccharide was determined to be an octasaccharide of the structure DeltaUAp2S(1-->[4)-alpha-D-GlcNpS6S(1-->4)-alpha-L-IdoAp2S(1-->](3)4)-alpha-D-GlcNpS6S by nuclear magnetic resonance spectroscopy, capillary electrophoresis, and polyacrylamide gel electrophoresis. Kinetic analysis of the interaction between the N-terminal apoE4 fragment and immobilized heparin by surface plasmon resonance yielded a K(d) of 150 nM. A similar binding constant (K(d) = 140 nM) was observed for the interaction between immobilized N-terminal apoE4 and the octasaccharide. Isothermal titration calorimetry revealed a K(d) of 75 nM for the interaction of the N-terminal apoE fragment and the octasaccharide with a binding stoichiometry of approximately 1:1. Using previous studies and molecular modeling, we propose a binding site for this octasaccharide in a basic residue-rich region of helix 4 of the N-terminal fragment. From the X-ray crystal structure of the N-terminal apoE4, we predicted that binding of the octasaccharide at this site would result in a change in intrinsic fluorescence. This prediction was confirmed experimentally by an observed increase in fluorescence intensity with octasaccharide binding corresponding to a K(d) of approximately 1 microM.

PubMed: 11258893
DOI: 10.1021/bi002417n

実験手法

X-RAY DIFFRACTION (2 Å)