1B4A

STRUCTURE OF THE ARGININE REPRESSOR FROM BACILLUS STEAROTHERMOPHILUS

1B4A の概要

• エントリーDOI: 10.2210/pdb1b4a/pdb
• 分子名称: ARGININE REPRESSOR (2 entities in total)
• 機能のキーワード: repressor, arginine, helix turn helix
• 由来する生物種: Geobacillus stearothermophilus
• 細胞内の位置: Cytoplasm (Probable): O31408
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 100857.27

構造登録者

Ni, J.,Sakanyan, V.,Charlier, D.,Glansdorff, N.,Van Duyne, G.D. (登録日: 1998-12-18, 公開日: 1999-06-15, 最終更新日: 2024-05-22)

主引用文献

Ni, J.,Sakanyan, V.,Charlier, D.,Glansdorff, N.,Van Duyne, G.D.
Structure of the arginine repressor from Bacillus stearothermophilus.
Nat.Struct.Biol., 6:427-432, 1999

PubMed Abstract: The arginine repressor (ArgR) is a hexameric DNA-binding protein that plays a multifunctional role in the bacterial cell. Here, we present the 2.5 A structure of apo-ArgR from Bacillus stearothermophilus and the 2.2 A structure of the hexameric ArgR oligomerization domain with bound arginine. This first view of intact ArgR reveals an approximately 32-symmetric hexamer of identical subunits, with six DNA-binding domains surrounding a central oligomeric core. The difference in quaternary organization of subunits in the arginine-bound and apo forms provides a possible explanation for poor operator binding by apo-ArgR and for high affinity binding in the presence of arginine.

PubMed: 10331868
DOI: 10.1038/8229

実験手法

X-RAY DIFFRACTION (2.5 Å)