1B0W

Structural comparison of amyloidogenic light chain dimer in two crystal forms with nonamyloidogenic counterparts

1B0W の概要

• エントリーDOI: 10.2210/pdb1b0w/pdb
• 関連するPDBエントリー: 1BRE
• 分子名称: BENCE-JONES KAPPA I PROTEIN BRE (2 entities in total)
• 機能のキーワード: immunoglobulin, amyloid, immune system
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Secreted : P01594
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 35835.37

構造登録者

Schormann, N.,Benson, M.D. (登録日: 1998-11-13, 公開日: 1998-11-16, 最終更新日: 2024-10-16)

主引用文献

Schormann, N.,Murrell, J.R.,Benson, M.D.
Tertiary structures of amyloidogenic and non-amyloidogenic transthyretin variants: new model for amyloid fibril formation
Amyloid, 5:175-187, 1998

PubMed Abstract: The most common form of hereditary systemic amyloidosis is familial amyloidotic polyneuropathy associated with single amino acid changes in the plasma protein transthyretin. So far, high resolution structures of only three amyloidogenic variants (Met30, Ser84, Ile122) and one non-amyloidogenic variant (Thr109) have been reported complemented by X-ray fiber diffraction studies and image reconstruction from electron micrographs of amyloid fibrils. To investigate the role of structural factors in this disease, we extended our studies to other transthyretin variants. We report crystallization and structural investigations of three amyloidogenic (Arg10, Ala60, Tyr77) and two non-amyloidogenic variants (Ser6, Met119). The similarity of these structures to normal transthyretin does not give direct clues to the fibril forming process. Since transthyretin amyloid fibrils contain a major fragment starting at position 49, besides the intact molecule, we calculated the solvent accessibility of residue 48. Indeed, all amyloidogenic variants show an increased main chain solvent exposure when compared to normal transthyretin and non-amyloidogenic variants, which can be postulated to result in increased susceptibility to proteolysis. After limited proteolysis, dimers are incapable of reassociation to native tetramers. We present a model for amyloid fibril formation based on formation of fibrils from N-terminal truncated dimers as building blocks.

PubMed: 9818054

実験手法

X-RAY DIFFRACTION (1.8 Å)