1AZS

COMPLEX OF GS-ALPHA WITH THE CATALYTIC DOMAINS OF MAMMALIAN ADENYLYL CYCLASE

1AZS の概要

• エントリーDOI: 10.2210/pdb1azs/pdb
• 分子名称: VC1, IIC2, GS-ALPHA, ... (7 entities in total)
• 機能のキーワード: complex (lyase-hydrolase), hydrolase, signal transducing protein, cyclase, effector enzyme, complex (lyase-hydrolase) complex, complex (lyase/hydrolase)
• 由来する生物種: Canis lupus familiaris (dog); 詳細
• 細胞内の位置: Membrane; Multi-pass membrane protein: P30803 P26769; Cell membrane; Lipid-anchor (By similarity): P04896
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 96554.63

構造登録者

Tesmer, J.J.G.,Sprang, S.R. (登録日: 1997-11-20, 公開日: 1998-02-25, 最終更新日: 2024-05-22)

主引用文献

Tesmer, J.J.,Sunahara, R.K.,Gilman, A.G.,Sprang, S.R.
Crystal structure of the catalytic domains of adenylyl cyclase in a complex with Gsalpha.GTPgammaS.
Science, 278:1907-1916, 1997

PubMed Abstract: The crystal structure of a soluble, catalytically active form of adenylyl cyclase in a complex with its stimulatory heterotrimeric G protein alpha subunit (Gsalpha) and forskolin was determined to a resolution of 2.3 angstroms. When P-site inhibitors were soaked into native crystals of the complex, the active site of adenylyl cyclase was located and structural elements important for substrate recognition and catalysis were identified. On the basis of these and other structures, a molecular mechanism is proposed for the activation of adenylyl cyclase by Gsalpha.

PubMed: 9417641
DOI: 10.1126/science.278.5345.1907

実験手法

X-RAY DIFFRACTION (2.3 Å)